华西虚拟期刊

华西虚拟期刊

查看全文

Recombinant adeno-associated virus (rAAV) is an attractive tool for basic science and translational medicine including gene therapy, due to the versatility in its cell and organ transduction. Previous work indicates that rAAV transduction patterns are highly dependent on route of administration. Based on this relationship, we hypothesized that intraperitoneal (IP) administration of rAAV produces unique patterns of tissue tropism. To test this hypothesis, we investigated the transduction efficiency of 12 rAAV serotypes carrying an enhanced green fluorescent protein (EGFP) reporter gene in a panel of 12 organs after IP injection. Our data suggest that IP administration emphasizes transduction patterns that are different from previously reported intravascular delivery methods. Using this approach, rAAV efficiently transduces the liver, pancreas, skeletal muscle, heart and diaphragm without causing significant histopathological changes. Of note, rAAVrh.10 showed excellent muscle transduction following IP administration, highlighting its potential as a new muscle-targeting vector.

Key words: HUMAN GENE-THERAPY; IMMUNE-RESPONSE; DEFICIENT MICE; VIRAL VECTORS; AAV SEROTYPES; EXPRESSION; INJECTION; LIVER; TRANSDUCTION; ROUTES

引用本文: . . 华西虚拟期刊, 2000, 1(1): -. doi: 10.1038/srep40336 复制

登录后 ,请手动点击刷新查看全文内容。 没有账号,