中华眼底病杂志

中华眼底病杂志

雷公藤红素对交感性眼炎患者外周血单个核细胞分泌白细胞介素17的抑制作用

查看全文

目的 观察雷公藤红素对交感性眼炎(SO)患者外周血单个核细胞(PBMCs)分泌白细胞介素(IL)-17的抑制作用,初步探讨其可能的机制。 方法 SO患者10例及健康对照组10名纳入研究。抽取所有参与者的静脉血,采用密度梯度离心法分离出PBMCs。将PBMCs分为A~D组。A组为正常人PBMCs;B组为SO患者PBMCs;C组为SO患者PBMCs,并在其培养液中加入0.5 μmol/L的雷公藤红素;D组为SO患者PBMCs,并在其培养液中加入1.0 μmol/L的雷公藤红素。培养3 d后,分别收集4组细胞的培养上清液,采用酶联免疫吸附测定法(ELISA)检测IL-23和IL-17的含量。向A、B组加入50 ng/ml 重组人IL-23(rIL-23)分别作为A1、B1组,加入50 ng/ml rIL-23+1 μmol/L 雷公藤红素分别作为A2、B2组。培养3 d后,分别收集4组细胞的培养上清液,采用ELISA检测IL-17的含量。 结果 A~D组PBMCs细胞培养上清液中IL-23含量分别为(228.43±17.27)、(513.85±36.46)、(381.07±20.93)、(237.14±17.97)pg/ml,IL-17含量分别为(220.55±31.15)、(866.77±72.92)、(517.43±54.87)、(242.89±34.09)pg/ml。除A组与D组PBMCs细胞培养上清液中IL-23含量之间差异无统计学意义外(P>0.05),其余两组间PBMCs细胞培养上清液中IL-23含量的差异均有统计学意义(P<0.05)。A1、A2、B1、B2组PBMCs细胞培养上清液中IL-17含量分别为(428.43±24.53)、(229.15±23.28)、(1373.39±89.51)、(571.01±94.88)pg/ml。A2、B2组PBMCs细胞培养上清液中IL-17含量分别较A1、B1组明显降低,差异均有统计学意义(P<0.05)。 结论 雷公藤红素可以抑制SO患者PBMCs分泌IL-17,其机制可能与抑制IL-23/IL-17通路发挥作用有关。

Objective To observe the effect of celastrol on the secretion of interleukin (IL)-17 in peripheral blood mononuclear cells in patients with sympathetic ophthalmia (SO), and its possible mechanisms. Methods Venous blood samples were extracted from 10 cases of sympathetic ophthalmia patients and 10 health objectives. The peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation and then were divided into 4 groups. Group A (control group): PBMCs of health objectives; Group B: PBMCs of SO patients; Group C: PBMCs of SO patients with 0.5 μmol/L celastrol in the medium; Group D: PBMCs of SO patients with 1 μmol/L celastrol in the medium. After culturing the cells for 3 days, the supernatant of 4 groups was collected, and the levels of IL-23 and IL-17 were detected by enzyme-linked immuno sorbent assay (ELISA). Then, the 50 ng/ml rIL-23 was added into the medium of group A which was the group A1; the 50ng/ml rIL-23 and 1 μmol/L Cela were added into to the medium of group A which was the group A2. For the medium of group B, the 50 ng/ml rIL-23 was added into the medium which was the group B1; the 50 ng/ml rIL-23 and 1 μmol/L celastrol were added into to the medium of group B which was the group B2. After culturing for 3 days, the supernatant of cells of these 4 groups was collected, and the levels of IL-17 were detected by ELISA. Results In group A, the levels of IL-23 and IL-17 were (228.43±17.27) pg/ml and (220.55±31.15) pg/ml respectively. In group B, the levels of IL-23 and IL-17 were (513.85±36.46) pg/ml and (866.77±72.92) pg/ml respectively. In group C, the levels of IL-23 and IL-17 were (381.07±20.93) pg/ml and (517.43±54.87) pg/ml respectively. In group D, the levels of IL-23 and IL-17 were (237.14±17.97) pg/ml and (242.89±34.09) pg/ml respectively. Between group A and D, there was no statistically significant difference in IL-23 or IL-17 level (P>0.05); but when comparing other groups, the differences were statistically significant (P<0.05). The levels of IL-17 in group A1 and group A2 were (428.43±24.53) pg/ml and (229.15±23.28) pg/ml and the difference was statistically significant (P<0.05). The levels of IL-17 in group B1 and group B2 were (1373.39±89.51) pg/ml and (571.01±94.88) pg/ml and the difference was statistically significant (P<0.05). Conclusion Celastrol can inhibit the secretion of IL-17 by PBMCs in SO patients via inhibiting the secretion of IL-23.

关键词: 眼炎,交感性/药物疗法; 雷公藤属; 白细胞介素17; 白细胞介素23

Key words: Ophthalmia, sympathetic/drug therapy; Tripterygium; Interleukin-17; Interleukin-23

引用本文: 秦廷玉, 高莎莎, 王文战. 雷公藤红素对交感性眼炎患者外周血单个核细胞分泌白细胞介素17的抑制作用. 中华眼底病杂志, 2018, 34(1): 51-54. doi: 10.3760/cma.j.issn.1005-1015.2018.01.013 复制

登录后 ,请手动点击刷新查看全文内容。 没有账号,
登录后 ,请手动点击刷新查看图表内容。 没有账号,
1. Chaithanyaa N, Devireddy SK, Kishore Kumar RV, et al. Sympathetic ophthalmia: a review of literature [J]. Oral Surg Oral Med Oral Pathol Oral Radiol, 2012, 113(2): 172-176. DOI: 10.1016/j.tripleo.2011.01.036.
2. 张锐, 钱江, 袁一飞. 交感性眼炎患者房水和血清细胞因子水平的分析 [J]. 中国眼耳鼻喉科杂志, 2014, 14(1): 17-20.Zhang R, Qian J, Yuan YF. Cytokine analysis in the aqueous humor and serum of patients with sympathetic ophthalmia [J]. Chin J Ophthalmol and Otorhinolaryngol, 2014, 14(1): 17-20.
3. Furusato E, Shen D, Cao X, et al. Inflammatory cytokine and chemokine expression in sympathetic ophthalmia: a pilot study [J]. Histol Histopathol, 2011, 26(9): 1145-1151. DOI: 10.14670/HH-26.1145.
4. 施沛青, 朱书, 钱友存. IL-17的信号传导及功能研究 [J]. 中国细胞生物学学报, 2011, 33(4): 345-357.Shi PQ, Zhu S, Qian YC. IL-17 signaling and function [J]. Chin J of Cell Bio, 2011, 33(4): 345-357.
5. Bettelli E, Korn T, Oukka M, et al. Induction and effector functions of T(H)17 cells [J]. Nature, 2008, 453(7198): 1051-1057. DOI: 10.1038/nature07036.
6. 李婷, 孙万邦. 眼外伤患者血清Th亚群细胞因子变化的临床意义 [J]. 中华眼外伤职业眼病杂志, 2016, 38(7): 494-497. DOI: 10.3760/cma.j.issn.2095-1477.2016.07.004.Li T, Sun WB. The clinical significance of the changes of Th subgroup cytokines in patients with ocular trauma [J]. Chin J Ocul Traum Occupat Eye Dis, 2016, 38(7): 494-497. DOI: 10.3760/cma.j.issn.2095-1477.2016.07.004.
7. Veldhoen M, Hocking RJ, Atkins CJ, et al. TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells [J]. Immunity, 2006, 24(2): 179-189. DOI: 10.1016/j.immuni.2006.01.001.
8. Bettelli E, Kuchroo VK. IL-12- and IL-23-induced T helper cell subsets: birds of the same feather flock together [J]. J Exp Med, 2005, 201(2): 169-171. DOI: 10.1084/jem.20042279.
9. Aggarwal S, Ghilardi N, Xie MH, et al. Interleukin-23 promotes a distinct CD4 T cell activation state characterized by the production of interleukin-17 [J]. J Biol Chem, 2003, 278(3): 1910-1914. DOI: 10.1074/jbc.M207577200.
10. McKenzie BS, Kastelein RA, Cua DJ. Understanding the IL-23-IL-17 immune pathway [J]. Trends Immunol, 2006, 27(1): 17-23. DOI: 10.1016/j.it.2005.10.003.
11. 陈建华. 炎症性肠病患者血清IL-23, IL-17, IL-25含量及临床意义 [J]. 中华临床医师杂志, 2012, 6(3): 698-699. DOI: 10.3877/cma.j.issn.1674-0785.2012.03.040.Chen JH. Serum IL-23, IL-17, IL-25 content and these clinical significance in patients with inflammatory bowel disease [J]. Chin J Clinicians, 2012, 6(3): 698-699. DOI: 10.3877/cma.j.issn.1674-0785.2012.03.040.
12. Chi W, Yang P, Li B, et al. IL-23 promotes CD4+ T cells to produce IL-17 in Vogt-Koyanagi-Harada disease [J]. J Allergy Clin Immunol, 2007, 119(5): 1218-1224. DOI: 10.1016/j.jaci.2007.01.010.
13. Chi W, Zhu X, Yang P, et al. Upregulated IL-23 and IL-17 in Behcet patients with active uveitis [J]. Invest Ophthalmol Vis Sci, 2008, 49(7): 3058-3064. DOI: 10.1167/iovs.07-1390.
14. 赵莹莲. 雷公藤红素作用机制的研究进展 [J]. 临床合理用药, 2015, 8(9): 170-171. DOI: 10.15887/j.cnki.13-1389/r.2015.26.124.Zhao YL. Research progress of the effect mechanism of tripterine [J]. Chin J of Clinical Rational Drug Use, 2015, 8(9): 170-171. DOI: 10.15887/j.cnki.13-1389/r.2015.26.124.
15. 罗丽娟, 张荷珍, 韩英军. 交感性眼炎的临床分析 [J]. 中华眼外伤职业眼病杂志, 2013, 35(4): 289-291. DOI: 10.3760/cma.j.issn. 2095-1477.2013.04.017.Luo LJ, Zhang HZ, Han YJ. Clinical analysis of sympathetic ophthalmia [J]. Chin J Ocul Traum Occupat Eye Dis, 2013, 35(4): 289-291. DOI: 10.3760/cma.j.issn. 2095-1477.2013.04.017.
16. 白雪莲. 33例多种交感性眼炎的临床诊断及治疗 [J]. 中国现代医生, 2009, 47(22): 128, 134. DOI: 10.3969/j.issn.1673-9701.2009.22.077.Bai XL. The clinical diagnosis and treatment of 33 cases of sympathetic ophthalmia. Chin Modern Doctor, 2009, 47(22): 128, 134. DOI: 10.3969/j.issn.1673-9701.2009.22.077.
17. 夏玲红, 崔岚. 雷公藤红素的药理作用研究进展 [J]. 医药导报, 2009, 28(6): 730-732. DOI: 10.3870/yydb.2009.06.020.Xia LH, Cui L. Research progress on pharmacological action of celastrol [J]. Herald of Medic, 2009, 28(6): 730-732. DOI: 10.3870/yydb.2009.06.020.